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Cimino,Cecilia; Vairo Cavalli,Sandra; Spina,Francisco; Natalucci,Claudia; Priolo,Nora. |
The objective of this work was the optimization of the conditions of in vitro culture for callus production of Silybum marianum (L.) Gaertn. (Asteraceae). Sections of cotyledons, previously disinfected by washing successively with ethanol 70º, NaClO (10% w/v) and Tween 20 (0.05% v/v) and rinsing with sterile distilled water, were used as explants. For its initial culture, B5 medium supplemented with BA and 2,4-D solidified with phytagel was used, and a 63% survival was achieved. To obtain callus, two solid media were assayed (S1 and S2) using B5 medium supplemented with growth regulators (BA and 2,4-D or NAA and BA, respectively). The calli were grown at 25ºC during 45 days in darkness. Growth kinetics was studied using S1 medium... |
Tipo: Journal article |
Palavras-chave: Asteraceae; Callus; Rhizogenesis; Silybum marianum. |
Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300012 |
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Estay,Carmen; Cautín,Ricardo; Neaman,Alexander; Castro,Monica. |
The etiolation and layering technique that is used on avocados has two critical steps, namely, the rooting of clonal rootstock and graft union formation between the commercial variety and clonal rootstock. This study analyzed the rooting step of the clonal rootstock regarding its influence on graft union formation and development. The optimum times for grafting the commercial variety onto clonal avocado rootstock were determined under two greenhouse conditions, heating and no heating. The results showed that under greenhouse conditions without heating, there was higher survival (%) in plants that were grafted 60 days after the rooting steps in the clonal scion, and, in the experiments in a heated greenhouse, the highest survival rate (%) was observed in... |
Tipo: Journal article |
Palavras-chave: Grafting; Histological cross section; Persea americana Mill; Rhizogenesis. |
Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000200006 |
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Santelices,Rómulo; Palfner,Götz. |
Hazelnut (Corylus avellana L.) is commonly used as mycorrhizal host for Tuber melanosporum Vitt. (black truffle). Black truffle orchards have been established in and outside its natural distribution area, currently also in Chile. Hazelnut can be propagated rapidly by cuttings, which, however, may not easily form mycorrhizal symbiosis under laboratory conditions. We successfully inoculated hazelnut cuttings with T. melanosporum and studied the effects of culture conditions, plant material and inoculum age on rhizogenesis and mycorrhization. Cuttings from sucker shoots showed the highest rooting rate (88.5%). Perlite and vermiculite as substrate increased root length (9.6 cm ). Treatment with auxin significantly increased root yield (up to 27 roots per... |
Tipo: Journal article |
Palavras-chave: Cuttings; Rhizogenesis; Mycorrhizae; Culture conditions. |
Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000200003 |
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Brondani, Gilvano Ebling; Escola Superior de Agricultura "Luiz de Queiroz" (ESALQ/USP); Wendling, Ivar; Empresa Brasileira de Pesquisa Agropecuária (Embrapa - Florestas); Brondani, André Ebling; Universidade Federal Fluminense; Araujo, Marla Alessandra; Universidade Federal de Lavras; Silva, André Luís Lopes da; Universidade Federal do Paraná; Gonçalves, Antonio Natal; Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo. |
It is possible to determine the optimum time for permanence of vegetative propagules (mini-cuttings) inside a greenhouse for rooting, and this value can be used to optimize the structure of the nursery. The aim of this study was to determine the dynamics of adventitious rooting in mini-cuttings of three clones of Eucalyptus benthamii x Eucalyptus dunnii. Sprouts of H12, H19 and H20 clones were collected from mini-stumps that were planted in gutters containing sand and grown in a semi-hydroponic system. The basal region of the mini-cuttings was immersed in 2,000 mg L-1 indole-3-butyric acid (IBA) solution for 10 seconds. The rooting percentage of the mini-cuttings, the total length of the root system and the rooting rate per mini-cutting were also evaluated... |
Tipo: Pesquisa |
Palavras-chave: 5.01.03.04-0 vegetative propagation; Clonal forestry; Organogenesis; Mathematical modeling; Rhizogenesis; Clonal mini-garden 5.01.03.04-0. |
Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/13059 |
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Rosa,Danimar Dalla; Tres,Gessika; Broti-Rissato,Bruna; Lorenzetti,Eloisa; Guimarães,Vandeir Francisco; Feiden,Armin. |
Abstract Platanus, an arboreal and deciduous plant, is widely adapted and can be used for several purposes. Despite producing viable seeds, production of platanus seedlings usually occurs through vegetative propagation; cuttings are the best and most efficient source for obtaining seedlings. Although cuttings offer a practical and easy method to obtain seedlings in different vegetable species, they are influenced by several factors, both external and internal. The present study aimed to analyze the behavior of plantain cuttings planted in sand subjected to damage or no damage at the cuttings base, and planted at depths of 20 and 40 cm. Experiment was carried out at the experimental station of horticulture and protected cultivation of UNIOESTE-Brazil, in a... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plant propagation; Propagation by cuttings; Propagation materials; Rooting potential; Rhizogenesis; Vegetative development. |
Ano: 2018 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122018000100109 |
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